To investigate the mechanism of action of Femarelle^® (DT56a) in promoting bone mineral density, osteoblast activation (which stimulates new bone formation) was examined in human bone cultures of pre- and post menopause females by the use of three markers:

• DNA synthesis: a marker of increased activity of osteoblasts through cell proliferation;
• Alkaline phosphatase (ALP): a marker of osteoblast activation;
• Creatine Kinase (CK) activity: a marker of estrogen receptor activation, which induces osteoblast activity.

These three markers were assessed in human bone cells treated either with E₂ or with Femarelle^®.

Results

Femarelle^® stimulated DNA synthesis, ALP and CK activity in both in pre- and post menopause female human bone cells; the optimal dose was seen at equivalent dosage of 2 capsules of Femarelle/day.

Activation of osteoblasts in human bone tissue culture

Conclusion

Femarelle^® stimulates osteoblast activity, indicating that, because of its bone-forming properties, Femarelle^® is a unique agent for prevention of post-menopausal bone loss.

Publication

J. of Steroid Biochemistry & Molecular Biology 2006;98(1):90-96: “DT56a Stimulates Gender-Specific Human Cultured Bone Cells In Vitro”; Somjen D., Katzburg S. Lieberherr M., Hendel D. and Yoles I.